Evaluation of Anti-Cataractogenic Activity of Spinacia Oleracea On Glucose - Induced Cataract in Isolated Goat Lens.

[摘要] Cataract, a leading cause of poor vision and blindness worldwide is recognized as a major complicationof diabetes mellitus and hyperglycemia. Increasing evidence that oxidative stress plays a role in cataractogenesishas led to the development of antioxidant nutrients from plant and synthetic origins that could be effective inpreventing cataract formation. The present study evaluated the in vitro anticataract potential of Spinaciaoleracea leaf extract against glucose-induced cataractogenesis in goat lenses. Freshly isolated goat lens wasdivided into the following five experimental groups and incubated for 72 h:55 mM glucose alone (Group I), 100μg/ml SE+55 mM glucose (Group II), 300 μg/ml SE+55mM glucose (Group III), 500 μg/ml SE+55mM glucose(Group IV) and 12 ng/ml Enalapril+55mM glucose. On photographic evaluation of lenses, a reduction in theopacity of the lens incubated with the plant extract was observed. After 72 h incubation, various biochemical2+ parameters such as total protein, H O , malondialdehyde (MDA), reduced glutathione (GSH) and Cu -induced2 2 lipoprotein dienes; enzymatic antioxidants like glutathione reductase (GR) and peroxidase (POX) were measuredin the lens homogenate. Thus, the leaf extract of spinach (100 μg/ml SE) protected the lens against glucose- induced oxidative damage which might help delay the progression of cataract.

[学科分类] 工程和技术（综合）

[关键词] antioxidant;cataract;hyperglycemia;goat lenses;spinach.