Cleaved Amplified Polymorphic Sequences (CAPS) Of Bacillus Cereus And Mutagenic Effect Of Acridine Orange On Alpha Amylase Production.

[摘要] The current study aimed to isolate and characterize local isolates from Egyptian soils using 16s rRNAtechnique and improve α-amylase enzyme production by Acridine orange (AO) as a mutagenic agent. The resultsof molecular characterization dependent on 16S rRNA using NCBI BLAST search assures that these isolates wereclosely related to the Bacillus cereus. Mutagenesis by (AO) concentration 0, 10, 20, 30, 40, 50, 60 and 70 µg/mLwas used for induction a mutation from Bacillus cereus. Alpha amylase production was determined in wild typeand its mutants. Sixteen mutants gave relatively better amylase production than wild type. These mutantscould be used in industries for the efficient conversion of starch to oligosaccharides. Cleaved AmplifiedPolymorphic Sequence (CAPS) for the analysis of genetic marker of 16S rRNA gene of Bacillus cereus and mutantswith HaeIII enzyme has doneand show the different fragments in mutants comparing with the wild type. Theseresults gave that this technique PCR doubled with restriction enzyme analysis (CAPS) may be used to morequickly analyses the results for detection and identify the bacterial.

[学科分类] 工程和技术（综合）

[关键词] 16S rRNA;HaeIII;CAPS;AO.