Study Of Active Constituents From Methanolic Extract Of Watermelon Fruit Extract; A Comparative Analysis Of Hybrid And Wild Cultivar.

[摘要] Plants are rich in active bio-molecules, and these molecules synthesized as secondary metabolites fora wide range of physiological functions, including defense and food reserve. These plant phytochemicalsbelong to a wide range of categories, including steroids, alkaloids, phenolic compounds, terpenes, saponin,and resin, etc. Watermelon is fruit for the richest source of various bio-active compounds, including vitaminsand minerals. Over a period of time numerous hybrid cultivars were designed for commercial and large scalecultivation of watermelon. The key question arises among scientific platform for the active phytochemicalspresent in the watermelon hybrid cultivar over wild type. The present study was aimed to carry out acomparative analysis of the presence of various active biomolecules in watermelon Citrulus Lanatus hybrid andwild type. We have extracted active phytochemicals using multiple solvents, including methanol, acetylacetate, and chloroform. The methanolic extract was further characterized for active phytochemicals using GCMS for both hybrid and wild type cultivar. Based on phytochemical analysis and preliminary activity analysis,i.e., the antimicrobial and antioxidant methanolic extract does possess a comparatively higher biologicalactivity and hence active biomolecules. The GC-MS analysis shows a wide range of phytochemicals, includingAlkaloids, Phenolic compounds, Saponin, Quinone, and Coumarin in methanolic extract. On the contrary,methanolic extract of wild type cultivar does possess flavonoid, saponnin and steroid, phenolic acids and glycoconjugates, flavonoids and glyco-conjugates, metabolites of phenylpropanoid pathway, such as gingerol,cinnamic acid, p-coumaric acid, etc.; organic acids such as citric acid, iso-citrate, fumarate, etc.; carotenoidsuch as beta-carotene metabolism products, curcuminoids; curcubitacin E, monosaccharaides andoligosaccharides, amino acids such as Citrulline, saturated mono and polyunsaturated fatty acids, andcompound lipids such as Ceramide and Sphinganine; nucleotide bases such as guanosine, uridine, etc. Thestudy shows watermelon hybrid cultivar are rich in diverse phytochemical over wild type cultivar preciselymethanolic extract.

[学科分类] 工程和技术（综合）

[关键词] GCMS;Watermelon;phytochemicals;extract methanol and biomolecules;Hybrid and wild typecultivar.