Acute Toxicity Evaluation of Temulawak (Curcuma xanthorrhiza Roxb) Hepatoprotective Supplement.

[摘要] This study was carried out to evaluate the acute toxicity of Temulawak (Curcuma xanthorrhiza Roxb)hepatoprotective supplement. Temulawak is a native Indonesian plant which known to have manypharmacological benefits. In vivo study was conducted in Wistar Rats by oral administration. Temulawak(Curcuma xanthorrhiza Roxb) hepatoprotective supplement was given at doses of 2000 mg/kg body weight(BW). Animals were observed for any toxic sign and symptoms during the first 24 hours especially in the first 4hours. In the absence of animal mortality, the observation is continued for up to 14 days with periodicobservations every day. At completion of study animals were sacrificed andmacroscopic observation wasdone. No mortality and clinical signs of toxicity were found in Temulawak (Curcuma xanthorrhiza Roxb)hepatoprotective supplement administered groups. The Lethal Dose 50 (LD 50) of this hepatoprotectivesupplement infemale and maleratsbelongs to the unclassified category according to Organization ofEconomic Cooperation and Development (OECD) guideline or at leastpractically non-toxic (LD50> 5000 mg/kgBW) which is equivalent to a dose of 56 000 mg (56 g) in humans (70 kg). From macroscopic observation, therewere no significant abnormalities on important organs (lung, heart, liver, kidney, stomach) after theadministration of this supplement at 2000 mg/kg BW. The oral LD50 of Temulawak (Curcuma xanthorrhizaRoxb) hepatoprotective supplement was greater than 5000 mg/kg BW thus it could regarded as non-toxic.Based on the results can be concluded the safety of Temulawak (Curcuma xanthorrhiza Roxb)hepatoprotective supplement in the animal tested.

[学科分类] 工程和技术（综合）

[关键词] acute toxicity;Curcuma xanthorrhiza Roxb;in vivo;LD50;supplement