Ameliorative Effect of Moringa Seed Oil Against Chlorambucil-Induced DNA Damage and Hepatotoxicity in Adult Male Albino Rats

[摘要] Moringa oil was potentially effective therapy in the treatment of xenobiotic-induced liverdisorders. The present study was carried out to investigate the biological effects of moringa oil againsthepatic injury and DNA damage induced by chlorambucil in rats. Forty male rats were divided intofour groups.Group I: rats maintained as control, group II: rats received moringa oil, group III: ratsreceived chlorambucil, group IV: rats received chlorambucil and moringa oil. At the end of the fourthweek, data showed that oral administration of chlorambucil significantly increased (p<0.001) bothcholesterol and triglycerides levels when compared with the control rats. Histopathological resultsshowed that chlorambucil produced cellular damage and severe inflammatory lesions in the livercompared to the control group. Ultrastructural examination of the liver revealed many changes.Pyknotic nuclei were seen and the majority of mitochondria had excessively dense matrices whilerough endoplasmic reticulum showed disorganization and dilation of their cisternae. Cytogenetically,hepatocytes of chlorambucil treated rats revealed significant DNA damage. Administration of moringaoil alongside with chlorambucil significantly reversed chlorambucil-induced toxicity in the hepatictissue. These data demonstrate that moringa oil exhibits potent hepatoprotective effects onchlorambucil-induced liver damage in rats through the prevention of DNA damage.

[学科分类] 工程和技术（综合）

[关键词] Chlorambucil;Moringa oil;Hepatotoxicity;Histopathology;Ultrastructure;DNA damage.