Effect Of Different Polyphenolic Fractions Of Lycium barbarum On The Expression Of DNMT1 And HDAC Gene.

[摘要] For a long time, the fruit of the Rubus species has been utilised as a meal in India.Polysaccharides, flavonoids, saponins, and alkaloids were isolated from the fruits and their contentsdetermined in order to assess their active components. The antioxidant and anticancer effects of the fourprimary ingredients were examined in vitro against Hela cells (Cervical Cancer Cell Line). Total flavonoidswere found to have significant antioxidant action. The highest phenolic content was observed inmethanolic extract of Lycium barbarum and Fragaria ananassa (Strawberry); 35.727937mg/gm and35.3007307 mg/gm respectively. The MTT assay was used to determine cytotoxicity in HeLa cells, andthe IC was reported in different fractions. The retention time was found to be maximum for Quercetin50 i.e., 18. 656. This was followed by HPLC analysis of fraction LBM70 for which reference gene for internalcontrol in this study was β-Actin. Hence our study is in accordance with the previous research whichclearly reveals that HDAC1 overexpression has been connected to a range of cancer prognoses. Theresults of this study reveal that treating Hela cells with a specific fraction of Lycium barbarum causesoverexpression of the DNMT1 and HDAC genes.

[学科分类] 工程和技术（综合）

[关键词] Flavanoids;Saponinns;DNMT1;HDAC;HPLC