In-vitro Anticariogenic Activity of P. betle Leaf Extract Against Oral Pathogens.

[摘要] The antimicrobial activity of leaf extract of Piper betle against oral bacteria that cause dental caries wasinvestigated in this report. Using agar-well diffusion method, the antimicrobial potential of the extract wastested on clinically isolated and reference strains, including Streptococcus mutans MTCC 497, Lactobacillusacidophilus MTCC 10307, Candida albicans MTCC 183, and Staphylococcus aureus MTCC 1144. The MIC thebacterial species were 0.16 mg/ml for all microorganisms tested. Subculturing microtiter wells that did notchange the colour of the indicator after incubation yielded the minimum bactericidal concentration (MBC). S.aureus was the most susceptible of all the bacteria examined. The ability of clinically isolated S. mutans todevelop a biofilm on the seashell surface was investigated further. In the presence of leaf extract of Piper betle,the biofilm reduction percent (RP%) assay was used to quantify the inhibition. the greatest reduction wasobserved at a concentration of 0.016 µg/ml of PE extract. From this we can conclude that extract of Piper betleleaves could be used as a phytotherapeutic agents to prevent biofilm formation by common oral pathogens.

[学科分类] 工程和技术（综合）

[关键词] Biofilm;Oral Pathogens;Streptococci;Piper betle