A Study Of Prevalence Of Urinary Isolates And Their Antimicrobial Susceptibility With Special Reference To Extended Spectrum Beta Lactamases Detection.

[摘要] Urinary tract infections (UTIs) are one of the most common infectious diseases encountered inthe clinical practice. Extended spectrum beta lactamases (ESBLs) production in gram negative bacteria,have emerged as a major problem in hospitalized as well as community based patients. Bacterialresistance to β-lactam antibiotics has risen dramatically, with ESBL contributing to this increase. Earlyidentification of ESBL production is becoming increasingly important. The objective of this study was todetermine the resistance patterns of the micro-organisms isolated from cases of UTI and to detect ESBLsproduction in gram negative bacteria. Uropathogens from symptomatic UTI cases were identified byconventional methods, over the period of eight months. Antimicrobial susceptibility testing wasperformed by Kirby Bauer's disc diffusion method. Gram negative isolates resistant to third generationcephalosporins were tested for ESBL production by two methods ( Modified Double disc synergy test(DDST) and Phenotypic confirmatory disc diffusion test (PCDDT)). Number of urinary isolates were 175over a study period of six months. E.coli was the predominant isolate 101 (57.7%). A total of 170 Gramnegative isolates resistant to third generation cephalosporins were tested for ESBL production. It wasobserved that ESBL production was present in13 isolates by DDST method and in 18 isolates by PCDDTisolates. Routine ESBL testing of all uropathogens along with conventional antimicrobial susceptibility isrecommended for all cases as this will help in the proper treatment of the patients and also preventfurther development of bacterial drug resistance.

[学科分类] 工程和技术（综合）

[关键词] Urinary tract infections;antimicrobial resistance;ESBLs.