Antibiotic Resistance Pattern OfE. Coli And Klebsiella Species Producing Extended Spectrum Beta Lactamase (ESBL) In Urinary Isolates: A Cross Sectional Study.

[摘要] Urinary tract infections (UTIs) are one of the most common infectious diseases encountered inthe clinical practice. Even if wide spread availability of antibiotics, UTI remains to be one of the mostcommon infectious diseases diagnosed. Further world wide data shows that there is an increasingresistance among UTI pathogens to routine antibiotics. Resistance have emerged even to newer and morepotent antimicrobial agents. Therefore, the aim of the present study is to determine the incidence ofextended spectrum beta – lactamase in urinary isolates of Escherichia coli (E. coli) and Klebsiella species.Urine samples from patients suspected to have UTI (Total of 542) were processed.Escherichia coli andKlebsiella species isolated in significant numbers were included. Standard microbiological proceduresand AST was done according to CLSI guidelines were followed for their identification. The isolates weretested for susceptibility to third generation cephalosporins and then for Double disc synergy test (DDST)& Phenotypic confirmatory disc diffusion test (PCDDT). Number of urinary isolates were 175 over a studyperiod of six months. E.coli was the predominant isolate 101 (57.7%) followed by Klebsiella species 49(28.33%) A total of 78 E.coli and Klebsiella species resistant to third generation cephalosporins weretested for ESBL production by two methods. ESBL production was seen in 18(10.28%) isolates. RoutineESBL testing of all uropathogens along with conventional antimicrobial susceptibility is recommended forall cases as this will help in the proper treatment of the patients and also prevent further development ofbacterial drug resistance.

[学科分类] 工程和技术（综合）

[关键词] Urinary tract infections;antimicrobial resistance;ESBLs