Antibacterial Activity of Biosynthesized Nano-Selenium and ItsEnvironmentalApplications

[摘要] Because the fast development of microbial resistance system, the finding of effective, ecofriendlyand environmental compatible agent is difficult and will be more difficult in the future, it is difficult butnot impossible. For that, the current research focused on production of nano-selenium by bacteria aseffective and environmental compatible antibacterial agent. Forty nine samples (soil, water, wastewaterand milk cracker) were collected from different locations in Delta region, Egypt. Hundred isolates ofbacteria were obtained from the collected samples on MSM medium supplemented with sodium seleniteand screened for selenium reduction; about 74 positive bacterial isolates were selected. The screeningtechnology was developed to obtain the superior bacterial isolates for nano-selenium manufacturing.Three bacteria were finished these procedures as the best producers. The culture and environmentalconditions were optimized for the 3 strains to obtain active and high yield of nano-selenium. Theproduced amount of nano-selenium was characterized using TEM, XRD, EDAX and UV/VISspectrophometer techniques. The characterization technology confirmed producing selenium with nano- properties (15-30 nm with spherical shape). The antibacterial activity of the produced nano-seleniumwas evaluated against several bacterial pathogens and the MIC was calculated. The produced nano- selenium has high activity and low MIC with the most tested pathogens. For that, it can be applied asantibacterial agent for controlling the pathogens in hospital, agricultural fields and municipal wastewatertreatment units.

[学科分类] 工程和技术（综合）

[关键词] Nano-selenium;Bacterial Synthesis;Pathogens;Environmental Applications.