Phytochemicals Profile of Methanolic Extract of Withania somnifera (L.) Dunal roots by Validated HPLC Technique.

[摘要] To performs phytochemicals profile of methanolic extract of Withania somnifera (L.) Dunal rootsby validated HPLC technique. Methanolic Extract was subjected to HPLC investigation, the PhenomenexLuna C18 column (250 mm *4.6 mm, 5 m) was used, with a flow rate of 1.5 ml/min and a UV Wavelengthof 227. This Chromatographic study revealed the major Bio active Phytochemicals has found withRetention time of Withanoside IV (14.78), Physagulin D (15.96), 27-Hydroxywithanone (17.22),Withanoside V and Withanoside VI (19.43), Withaferin A (19.85), Withastramonolide (21.17),Withanolide A (22.04), Withanone (22.28), Withanolide B (25.36) are found in Retention Time rangebetween 14.78 to 25.36. linearity range wasplot between10 to 30 mg/ml , LOD was found to beapproximately 0.36 ppm and LOQ is 1.18 ppm, Intermediate Precision intraday shows 0.9 %, Interday is0.25 %, Average Recovery is94.38 % when planed at 3 different concentration level 50 %, 100 % and150 %. The result obtained from these studies shows the present HPLC method was accurate, Precise andValidate for Phytochemicals analysis and this approach is recommended for routine quality controlanalysis of Active Phytochemicals in a wide variety of herbal plants.

[学科分类] 工程和技术（综合）

[关键词] HPLC;Withania somnifera (L.) Dunal;Withanoside;Withanolide;Withaferin.