Characterization of hexose transporter genes in the views of the chronological life span and glucose uptake in fission yeast

[摘要] Fission yeast, Schizosaccharomyces pombe, possesseseight hexose transporters, Ght1~8. In order to clarify the role of each hexose transporter on glucoseuptake, a glucose uptake assay system was established and the actual glucose uptake activity of eachhexose transporter-deletion mutant was measured.Under normal growth condition containing 2%glucose, ∆ght5 and ∆ght2 mutants showed large andsmall decrease in glucose uptake activity, respectively. On the other hand, the other deletion mutantsdid not show any decrease in glucose uptake activity indicating that, in the presence of Ght5 and Ght2,the other hexose transporters do not play a significant role in glucose uptake. To understand therelevance between glucose uptake and lifespanregulation, we measured the chronological lifespanof each hexose transporter deletion mutant, andfound that only ∆ght5 mutant showed a significantlifespan extension. Based on these results we showedthat Ght5 is mainly involved in the glucose uptakein Schizosaccharomyces pombe, and suggested thatthe ∆ght5 mutant has prolonged lifespan due tophysiological changes similar to calorie restriction.

[学科分类] 微生物学和免疫学

[关键词] fission yeast;Ght5;hexose transporter;lifespan;Schizosaccharomyces pombe