Utilization of various carbon sources for poly(3-hydroxybutyrate) [P(3HB)] production by Cobetia sp. IU180733JP01 (5-11-6-3) which is capable of producing P(3HB) from alginate and waste seaweed

[摘要] The marine bacterium Cobetia sp. IU180733JP01(5-11-6-3) can accumulate poly(3-hydroxybutyrate)[P(3HB)] during cultivation on alginate or wasteLaminaria sp. Here, we examined this strain’s ability to utilize various carbon sources for P(3HB)production. When cultured in mineral salt mediumcontaining 1% (w/v) glucose, fructose, glycerol, orgluconic acid, the strain showed better growth andhigher P(3HB) production than on alginate, withfructose enabling the highest P(3HB) yield (0.8 ±0.06 g/L). We also predicted metabolic pathways forP(3HB) synthesis based on draft genome sequenceanalysis, in which carbon sources are assimilatedthrough Entner–Doudoroff and Embden–Meyerhofpathways, and the resultant acetyl-CoA is convertedinto P(3HB). Our findings reveal the potential ofthe 5-11-6-3 strain for application in bioplastic production from not only marine biomass but also otherbiomass and industrial wastes.

[学科分类] 微生物学和免疫学

[关键词] bioplastics;polyhydroxyalkanoate;biomass