Isolation and identification of Enterococcus gallinarum P581a, a strain of intestinal bacteria deglycosylating flavone C -glycosides

[摘要] Flavone C-glycosides are not easily degradedbecause of their strong C-C bond between sugarmoieties and aglycones. However, some bacteriasuch as intestinal species can produce specificenzymes to degrade them. In this study, a bacterialstrain P581a, which is capable of deglycosylatingflavone C-glycosides, was isolated from human intestinal bacteria and was identified as Enterococcusgallinarum by morphological examination, physiological and biochemical analysis and 16S rRNA genesequencing. This strain may produce a specific flavonoside glycosidase. The activity of the enzyme inthe culture medium containing different quantityof carbon sources was also studied, and it was foundthat the content of carbon sources is negatively correlated with the deglycosylation efficiency of thisstrain.

[学科分类] 微生物学和免疫学

[关键词] Enterococcus gallinarum;flavoneC-glycosides;deglycosylation;identification