Development of a microtiter plate-based analysis method of nitric oxide dioxygenase activity

[摘要] Nitric oxide (NO) functions in cell protection or celldeath, depending on its concentration. Therefore,regulation of the intracellular concentrations of NOby its degradation systems is important for cellularfunctions. One of the NO degrading enzymes, flavohemoglobin (FHb), which has NO dioxygenase(NOD) activity, is a promising target for antibiotics,based on the finding that FHb-deficient pathogensexhibited reduced host toxicity. Here, we developeda high-throughput method to measure the NODactivity. Our newly developed method could contribute to the screening of potential antibiotics withNOD inhibitory activity.

[学科分类] 微生物学和免疫学

[关键词] enzyme assay;fluorescence probe;microtiter plate;nitric oxide dioxygenase;Saccharomyces cerevisiae