A) polymorphism conferring high ethyl caprylate productivity in industrial sake yeast strains" >Genotypic analysis of the FAS2- F1279Y (3836T>A) polymorphism conferring high ethyl caprylate productivity in industrial sake yeast strains

[摘要] In Saccharomyces cerevisiae, ethyl caprylate is produced by the esterification of caprylic acid, whichis synthesized through the action of fatty acid synthase. A recent study reported a yeast mutant witha single nucleotide substitution in the alpha subunitof fatty acid synthase (FAS2) gene (F1279Y;3836T>A) that produced large amounts of ethylcaprylate. Here, we designed two primer sets (P1/P2 and P3/P4) with mismatches that incorporaterestriction sites for the enzymes NdeI and SspI,respectively and developed an easy and rapid polymerase chain reaction-restriction fragment lengthpolymorphism assay to identify yeasts harboringthe FAS2-F1279Y mutation associated with highethyl caprylate productivity.

[学科分类] 微生物学和免疫学

[关键词] polymerase chain reaction-restrictionfragment length polymorphism;Saccharomycescerevisiae;sake yeast