COVID-19 molecular level laboratory diagnoses

[摘要] AIMS: Aims: The Coronavirus Disease 2019 (COVID-19) caused not only global pandemic, but it also pointed at unprepared health care systems and countermeasures were introduced under the pressure of urgent circumstances. This review is focused on discussion and critical evaluation of instrumental tools for COVID-19 diagnosis that were developed in the last months. METHODS: Survey of actual literature and scientific reports was made. The most substantial analytical and diagnostical methods were identified and described. Principles and limitations of the methods are described, and actual papers are cited in this review. RESULTS: Analytical and diagnostical methods like Polymerase Chain Reaction (PCR), Loop-mediated isothermal Amplification (LAMP), Lateral Flow Immunochromatography Assay (LFIA), Enzyme-Linked Immunosorbent Assay (ELISA), biosensors and ChemiLuminescence ImmunoAssay (CLIA) are discussed for assay of viral particles, antigens and specific host antibodies in blood, serum, plasma, nasopharyngeal swab and other samples in order to diagnose COVID-19. CONCLUSIONS: The Coronavirus Disease 2019 (COVID-19) is an emerging disease that has spread over the world since the end of year 2019. The global epidemic pointed at the necessity to introduce sensitive methods for instrumental diagnosis of COVID-19 and distinguishing it from the other viral diseases. (Tab. 2, Ref. 96).

[学科分类] 医学（综合）

[关键词] antibody;biosensor;COVID-19;Coronavirus Disease 2019;diagnosis;enzyme-linked immunosorbent assay;ELISA;immunoassay;polymerase chain reaction;severe acute respiratory syndrome coronavirus 2;SARS-CoV-2