Updating the Impact of Lipid Metabolism Modulation and Lipidomic Profiling on Oocyte Cryopreservation

[摘要] Oocyte cryopreservation has drastically improved in recent years and is receiving widespread clinical use with increasing demand for fertility preservation and assisted reproduction treatments. However, there are still several points to be reviewed in terms of suppressing sub-lethal damages and improving overall safety, especially when trying to preserve oocytes at the germinal vesicle stage or oocytes matured in vitro . The lipid content of oocytes is highly associated with both their competence and cryotolerance. Differences in lipid content are observed not just between different species but also at different developmental stages and when the oocytes are kept under different conditions, including cryopreservation. Many efforts have been made to understand how physiological or in vitro alterations in the lipid profile of oocytes impacts cryotolerance and vice-versa; however, the dynamics of cytosolic and membrane lipid involvement in the cryopreservation process remains poorly clarified in the human female gamete. This review presents an updated overview of the current state of cryopreservation techniques and oocyte lipidomics and highlights possible ways to improve cryotolerance, focussing on lipid content modulation.

[学科分类] 经济地理

[关键词] Cryopreservation;lipid metabolism;lipid profile;mass spectrometry;oocyte