Generation of a homozygous ABCA7 -knockout human iPSC line using the CRISPR/Cas9 system

[摘要] ABCA7 encodes an ATP-binding cassette transporter, and its loss-of-function variants are associated with Alzheimer’s disease. To investigate the role of ABCA7 deficiency in the pathogenesis of Alzheimer’s disease, we generated a homozygous ABCA7 -knockout induced pluripotent stem cell (iPSC) line using CRISPR/Cas9-mediated gene editing. This ABCA7 -deficient iPSC line maintains a normal karyotype, expression of pluripotency markers, and trilineage differentiation capacity.

[学科分类] 生理学