Generation of a GFI1-flag knock-in human embryonic stem cell line using CRISPR-Cas9 technology

[摘要] GFI1 is a DNA binding transcriptional repressor, it is shown to be an important gene associated with blood cells development and many blood diseases (Möröy et al., 2015). But the role of GFI1 in human hematopoietic   development has not been known (Thambyrajah et al., 2016). To illustrate the function of GFI1 in human hematopoietic   development, we constructed a GFI1 -2 × flag-tag knock-in human embryonic stem cell line by CRISPR/Cas9 mediated gene targeting, and it would be the effective tool to study GFI1 . The cell line could express GFI1 -2 × flag-tag and can be identified with western blot and immunofluorescence. This cell line maintains stem cell morphology, and displays normal karyotype, pluripotent stem cell marker expression and differentiation potential.

[学科分类] 生理学