Generation of two PITX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9 system

[摘要] PITX2 is a homeobox gene located in the human 4q25 locus and is commonly associated with atrial fibrillation (AF). Here, we generated two PITX2 knock-out human induced pluripotent stem cell (iPSC) lines using CRISPR/Cas9 genome editing. The edited iPSCs maintained full   pluripotency, normal karyotype and spontaneous   differentiation capability. This cell line provides a suitable model for investigating the physiopathology   of PITX2 mutation in atrial fibrillation.

[学科分类] 生理学