Generation of homozygous Na v 1.8 knock-out iPSC lines by CRISPR Cas9 genome editing to investigate a potential new antiarrhythmic strategy

[摘要] The sodium channel Na v 1.8, encoded by SCN10A , is reported to contribute to arrhythmogenesis by inducing the late I Na and thereby enhanced persistent Na + current. However, its exact electrophysiological role in cardiomyocytes remains unclear. Here, we generated induced pluripotent stem cells (iPSCs) with a homozygous SCN10A knock-out from a healthy iPSC line by CRISPR Cas9 genome editing. The edited iPSCs maintained full pluripotency, genomic integrity, and spontaneous in vitro differentiation capacity. The iPSCs are able to differentiate into iPSC-cardiomyocytes, hence making it possible to investigate the role of Na v 1.8 in the heart.

[学科分类] 生理学