Generation of a human iPSC line (MPIi008-A) from a patient with Denys-Drash syndrome

[摘要] An induced pluripotent stem cell (hiPSC) line (MPIi008-A) was generated from fibroblasts of a 1-year-old male patient with Denys-Drash syndrome using lentiviral delivery of reprogramming factors OCT4, SOX2, KLF4 and c-MYC. The MPIi008-A iPSC line exhibited typical iPSC morphology and normal karyotype, expressed pluripotent stem cell markers, and showed developmental potential to differentiate into derivatives of all three germ layers in vivo . The hiPSC line harbours a heterozygous missense mutation (R394L) in exon 9 of the WT1 gene.

[学科分类] 生理学