The Effect of Chronic Kidney Disease Induction Upon Affective Factors on Muscle Apoptosis in Rats and the Role of Aerobic Training in Its Modulation

[摘要] Background and Objectives Chronic Kidney Disease (CKD) is characterized by progressive and irreversibleloss of renal function. One of the complications of this disease is muscle wasting and atrophy. The aimof this study was to determine the effect of exercise training on CKD-induced muscle apoptosis in rats.Subjects and Methods In the present developmental study, 30 rats were selected and divided into 3groups: disease training, disease control and healthy controls. Induction of CKD was through primaryand final 1.3 resection of one kidney and complete resection of the other kidney. The training programconsisted of eight weeks of continuous running training with an intensity of 45-55% VO2max. After removalof EDL muscle, protein level and Bcl-2 and Bax gene expression were measured by immunohistochemistryand real-time PCR, respectively.Results Induction of CKD caused a significant increase in protein level and gene expression of Bax andBax/Bcl-2 and a significant decrease in Bcl-2 compared to the healthy control group (P<0.001). Also significantdecrease was funded in the expression of genes and proteins Bax and Bax/Bcl-2 (P<0.001) anda significant increase was funded in protein Bcl-2 (P<0.001), without significant changes in the geneexpression of Bcl-2 (P=0.935) in disease training compared to the disease control.Conclusion The results of the present study support the anti-apoptotic effect of exercise on muscle tissueof CKD patients; therefore, it is recommended that clinical research be conducted in this regard forexercise recommendations for these patients.

[学科分类] 基础医学

[关键词] Chronic kidney disease;Apoptosis;Continuous Aerobic Training