The Effect of Aerobic Training with various intensity on TCF4/CHOP10 Complexes in Subcutaneous Fat Tissue of Male Wistar Rat

[摘要] Background and Objectives This study aims to evaluate the effect of different intensities of aerobic trainingon the expression of transcription factor 4 (TCF4) and C/EBP homologous protein (CHOP10) genes insubcutaneous adipose tissue of male Wistar rats.Subjects and Methods In this experimental study, 32 male Wistar rats with a mean age of 6 weeks anda weight of 237±33 gr were used. Then, they were randomly divided into four groups of 8, includingcontrol group, moderate-intensity aerobic training (MIT), high-intensity aerobic training (HIT), and highintensityinterval aerobic training (HIIT). The MIT group trained at an intensity of 65% VO2max for 47minutes. The HIT group running at a speed of 20 meters per minute with an increasing inclination for40 minutes. The HIIT group trained at an intensity of 90-100% VO2max for 37 minutes. Adipose tissuesample was collected 24 hours after the last training session to evaluate the expression of TCF4 andCHOP10 genes by real-time PCR method.Results There was a significant difference in the expression of CHOP10 gene in the subcutaneous tissueof male Wistar rats in the HIIT group compared to the MIT and control group (P=0.004). However, no significantdifference was observed between HIIT and HIT groups (P=1). In addition, there was a significantdifference in TCF4 gene expression in the HIIT group compared to the control group (P=0.006). However,no significant difference was observed between HIIT and HIT groups (P=1)Conclusion The expression level of adipogenic genes decreased after HIIT. These findings indicate thatthis exercise is effective in controlling the expression of genes involved in fat metabolism.

[学科分类] 基础医学

[关键词] Adipose tissue;transcription factor 4;endoplasmic reticulum stress;aerobic training