Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase (vol 401, pg 485, 1999)

[摘要] Mitochondrial preproteins are imported by a multisubunit translocase of the outer membrane (TOM), including receptor proteins and a general import pore(1-5). The central receptor Tom22 binds preproteins through both its cytosolic domain and its intermembrane space domain(6-10) and is stably associated with the channel protein Tom40 (refs 11-13). Here we report the unexpected observation that a yeast strain can survive without Tom22, although it is strongly reduced in growth and the import of mitochondrial proteins. Tom22 is a multifunctional protein that is required for the higher-level organization of the TOM machinery. In the absence of Tom22, the translocase dissociates into core complexes, representing the basic import units, but lacks a tight control of channel gating. The single membrane anchor of Tom22 is required for a stable interaction between the core complexes, whereas its cytosolic domain serves as docking point for the peripheral receptors Tom20 and Tom70. Thus a preprotein translocase can combine receptor functions with distinct organizing roles in a multidomain protein.

[关键词] OUTER-MEMBRANE PROTEIN;GENERAL IMPORT PORE;RECEPTOR COMPLEX;YEAST MITOCHONDRIA;NEUROSPORA-CRASSA;CELL VIABILITY;CHANNEL;COMPONENT;DOMAINS;MOM22