Isolation of RNA From Peripheral Blood Cells: A Validation Study for Molecular Diagnostics by Microarray and Kinetic RT-PCR Assays � Application in Aerospace Medicine

[摘要] Gene expression studies in clinical diagnostic settings involve a large number of samples collected at different time points requiring effective methods for collection, transportation, storage, and isolation of RNA to maintain the integrity of expression profiles.

[发布机构] FAA Office of Aerospace Medicine - Civil Aerospace Medical Institute

[关键词] RNA Isolation;Leukocytes;Human Whole Blood;RT-PCR;cDNA Microarray;Gel Electrophoresis;b-Actin;Cyclophilin;Gene Expression;PAXgeneTM Blood RNA