Is Increased Low-dose somatic Radiosensitivity Associated with Increased Transgenerational Germline Mutation

[摘要] Using single-molecule polymerase chain reaction, the frequency of spontaneous and radiation-induced mutation at an expanded simple tandem repeat (ESTR) locus was studied in DNA samples extracted from sperm and bone marrow of Atm knockout (Atm+/–) heterozygous male mice. The frequency of spontaneous mutation in sperm and bone marrow in Atm+/– males did not significantly differ from that in wild-type BALB/c mice. Acute gamma-ray exposure did not affect ESTR mutation frequency in bone marrow and resulted in similar increases in sperm samples taken from Atm+/– and BALB/c males. Taken together, these results suggest that the Atm haploinsufficiency analyzed in our study does not affect spontaneous and radiation-induced ESTR mutation frequency in mice.

[发布日期] 2008-10-02

[学科分类] 核能源与工程

[关键词] BONE MARROW;DNA;MALES;MICE;MUTATION FREQUENCY;MUTATIONS;POLYMERASE CHAIN REACTION;RADIOSENSITIVITY;SPERMATOZOA;SPONTANEOUS MUTATIONS Low-Dose;Radiosensitivity