Detection of BCR-ABL Fusion mRNA Using Reverse Transcriptase Loop-mediated Isothermal Amplification

[摘要] RT-PCR is commonly used for the detection of Bcr-Abl fusion transcripts in patients diagnosed with chronic myelogenous leukemia, CML. Two fusion transcripts predominate in CML, Br-Abl e13a2 and e14a2. They have developed reverse transcriptase isothermal loop-mediated amplification (RT-LAMP) assays to detect these two fusion transcripts along with the normal Bcr transcript.

[发布日期] 2011-12-08

[学科分类] 生物科学（综合）

[关键词] AMPLIFICATION;DETECTION;LEUKEMIA;MYELOID LEUKEMIA;PATIENTS