Isotope effects in gas–liquid chromatography of steroids

[摘要] When gas–liquid chromatography was performed with steroids containing H3 and C14, the H3/C14 ratio of the effluent was higher at the beginning and at the tail of the steroid peak. The H3/C14 ratio of the entire peak agreed with that determined by paper and thin-layer chromatography. Thus, gas–liquid chromatography on polar and nonpolar phases commonly used for chromatography of steroids apparently has sufficient resolving power to partially separate isotopic species whose molecular weights differ by 2% or less.

[关键词] gas–liquid chromatography;isotope separations;isotope ratios;steroids;doubly;labeled steroid derivatives