Δ8(9),22-Ergostadiene-3β-ol, an ergosterol precursor accumulated in wild-type and mutants of yeast

[摘要] Whereas wild-type strains of Saccharomyces cerevisiae can synthesize up to 7% dry weight of ergosterol, a polyene-resistant mutant has been obtained which produces no ergosterol. Instead, a C-28 methyl sterol is produced, and it has been identified as Δ8(9),22-ergostadiene-3β-ol. This sterol is converted to ergosterol by wild-type yeasts and is observed transiently in cells during aerobic adaption of anaerobically grown wild-type yeasts. The new sterol is proposed as an intermediate in ergosterol biosynthesis.

[关键词] ergosterol;sterol mutant;yeast sterol;polyene resistance