A simple lipase assay using trichloroacetic acid
[摘要] An extremely rapid and sensitive assay for lipoprotein lipase activity, suitable for routine determinations, is described. The substrate for the assay is emulsified [2-3H]glyceryl trioleate, activated by serum. The method is based on trichloroacetic acid precipitation of unreacted substrate and measurement of 3H-labeled glycerol.
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[关键词] adipose tissue;postheparin plasma;triolein;3H-labeled glycerol;lipoprotein lipase;triglyceride [时效性]
