As part of a regional monitoring program, water samples were collected in the San Francisco Bay estuary during 21 cruises from January through December 1996. Conductivity, temperature, light attenuation, turbidity, oxygen, and in-vivo chlorophyll fluorescence were measured longitudinally and vertically in the main channel of the estuary from south of the Dumbarton Bridge in the southern part of the Bay to Rio Vista on the Sacramento River. Discrete water samples were analyzed for chlorophyll a, phaeopigments, suspended participate matter, and dissolved oxygen. Water density was calculated from salinity, temperature, and pressure (depth), and is included in the data summaries.