Comparative Analysis of RNase P

[摘要] This Master's Thesis contains a description of the results of two research projects that were worked on over the course of two years.The first was an attempt to heterologously reconstitute the Bacillus subtilis RNase P RNA with the RNase P protein subunits of Methanothermobacter thermoautotrophicus, to identify a functional homology between one or more of the archaeal proteins with the Bacillus subtilis RNase P protein. Unfortunately, the reconstitution experiment could not be completed due to the poor quality of the pre-tRNAasp substrate synthesized.The second project dealt with comparative analysis of bacterial RNase P RNA.Eleven new RNase P RNA genes were amplified, sequenced, and their secondary structures derived by comparative analysis.

[发布机构] University:North Carolina State University

[效力级别] comparative analysis

[关键词] holoenzyme;comparative analysis;RNase P