Project 1 Molecular genetic analysis of m6A methylation of mRNA in a Drosophila Model Project 2 Development of a phenotypic highthroughput-screening assay to identify novel molecules that inhibit Mycobacteria

[摘要] Project 1:The genetic information on living systems is relayed from the DNA to protein through a chain of important processes. Various pathways regulate the process of mRNA synthesis into proteins among which methylation is an important one. Internal methylation has known regulate alternate splicing and regulate neuronal development. Knocking out genes involved in synthesis of proteins binding to methylated sites on the mRNA leads to an understanding of how the proteins with the modified mRNA modulate the neuronal development. This project utilizes Drosophila as a model organism to study the effects of the knock outs.Project 2: Tuberculosis and important disease, which has plagued mankind since centuries. With the development of modern drug regimens it was assumed that the disease is cured but with recent rise in drug resistant strains the current drug therapies are failing to control the spread of the disease. Combined with a high level of immunocompromised patients the bacilli has been able to invade further and increase the number of mortality rates. The current scenario of drug discovery involves highthroughput screening of new compounds and drugs, which are being synthesized, by chemists and pharamceuticals. The HTS methodology for screening requires a robust and reliable assay to give indications about new inhibitory compounds against Mycobacteria.

[发布机构] University:University of Birmingham;Department:School of Biosciences

[关键词] Q Science;QR Microbiology