Development of fluorophore-tagged DNA probes for cellular imaging applications

[摘要] Single nucleotide polymorphisms (SNPs) are single base variations in DNA which give genetic variation. However, SNPs can also be linked to the development of certain diseases. Modified oligonucleotides used to probe biological changes and processes have become an important focus of scientific research. Fluorescent tagging of DNA can be used to sense SNPs in DNA targets through differences in emission intensity on the formation of a duplex.An anthracene-tagged DNA probe developed by Tucker \(et\) \(al\). is able to discriminate between a fully complementary DNA target sequence and one with a single base difference. This thesis describes how SNP sensing with anthracene-tagged DNA has been extended to SNPs in RNA targets and sequences associated with Alzheimer's disease. Finally, a new dual fluorophore DNA probe was designed for SNP sensing via FRET.

[发布机构] University:University of Birmingham;Department:School of Chemistry

[关键词] Q Science;QD Chemistry