CaMK1D kinase: a potential target for breast cancer therapy

[摘要] Patients with breast cancer are currently offered cytotoxic treatments, which are associated with poor patient prognosis. Recent studies of the molecular basis of several breast cancers suggested a specific kinase which serves as a driver of disease progression. In-house testing of small molecule compounds using structure-based techniques have already exhibited selective inhibition of the kinase. Here, we developed a cell-based model system for assays required to evaluate these inhibitors in vitro. Cell lines were stably transformed using lentiviral transfections and western blot analysis of these cell lines identified potential biomarkers that could serve as a molecular readout of the inhibitor potency. Cytotoxicity and migration assays proposed that the primary inhibitor of interest does not specifically inhibit the kinase. Thus, the model system generated is appropriate for studying the preliminary in vitro effects of inhibition of this putative breast cancer target in cell-based assays.

[发布机构] University:University of Birmingham;Department:Institute of Cancer Studies

[关键词] R Medicine;RC Internal medicine;RC0254 Neoplasms. Tumors. Oncology (including Cancer)