Novel approaches for image analysis of in vitro epithelial cultures with application to silver nanoparticle toxicity

[摘要] A novel imaging approach was developed for the purpose of counting cells from phase contrast microscopy images of laboratory grown (in vitro} cultures of epithelial cells. Validation through comparison with standard laboratorycell counting techniques showed this approach provided consistent and comparableresults, whilst overcoming limitations of these existing techniques, such as operatorvariabilityand sample destruction. The imaging approach was subsequently applied to investigate the effects of silver nanoparticles (AgNP} on H400 oral keratinocytes. Concurrent investigationsinto antimicrobial effects of AgNP were performed on Escherichia coli, Staphylococcus aureus and Streptococcus mutans to providemodels for Gram-positive and Gram-negative infection, and to compare with the literature and oral keratinocyte toxicity.It was found that AgNP elicit size-, dose- and time-dependent growthinhibition in both human cells and bacteria, although bacterial inhibition was not achieved without significant cytotoxicity at the same concentrations.

[发布机构] University:University of Birmingham;Department:School of Chemistry

[关键词] Q Science;QD Chemistry