4D Biofabrication of Branching Multicellular Structures: A Morphogenesis Simulation Based on Turing's Reaction-Diffusion Dynamics

[摘要] The recently emerged four-dimensional (4D) biofabrication technique aims to create dynamic three-dimensional (3D) biological structures that can transform their shapes or functionalities with time when an external stimulus is imposed or when cell postprinting self-assembly occurs. The evolution of 3D pattern of branching geometry via self-assembly of cells is critical for 4D biofabrication of artificial organs or tissues with branched geometry. However, it is still unclear that how the formation and evolution of these branching pattern are biologically encoded. We study the 4D fabrication of lung branching structures utilizing a simulation model on the reaction-diffusion mechanism, which is established using partial differential equations of four variables, describing the reaction and diffusion process of morphogens with time during the development process of lung branching. The simulation results present the forming process of 3D branching pattern, and also interpret the behaviors of side branching and tip splitting as the stalk growing, through 3D visualization of numerical simulation.

[发布机构] College of Mechanical and Electrical Engineering, Hohai University, Changzhou, JIANGSU; 213022, China^1;Changzhou Key Laboratory of Digital Manufacture Technology, Hohai University, Changzhou, JIANGSU; 213022, China^2

[效力级别] 无线电电子学

[关键词] Biological structures;Branching structures;Development process;Formation and evolutions;Multicellular structure;Reaction-diffusion dynamics;Reaction-diffusion mechanisms;Threedimensional (3-d)