Colloidal quantum dots for fluorescent labels of proteins

[摘要] The work is devoted to the synthesis of colloidal quantum dots (QDs) and their bioconjugates with proteins. Various QDs were obtained as well with synthesis method in an organic solvent followed by hydrophilization and functionalization or synthesis in aqueous phase provides obtaining hydrophilic QDs directly. Particular attention is paid to the synthesis of QDs as fluorescent tags in the near infrared where minimum absorption occurs and the fluorescence of biological tissue and synthetic materials used in analytical systems. A method for the QDs synthesis of type fluorescent core/shell CdTeSe/CdS/CdZnS-PolyT with mixed telluride, selenide cadmium core with a high quantum yield and high resistance to photoaging. It is shown that these quantum dots may be effectively used in the immunoassay.

[发布机构] State University dubna, Universitetskaya St. 19, Dubna, Moscow region; 141982, Russia^1;Universidad Industrial de Santander, Carrera 27, Calle 9, Bucaramanga, Santander; 68001000, Colombia^2;Research Institute of Applied Acoustics, 9 Maya st. 7A, Dubna, Moscow region; 141980, Russia^3;National Research Center kurchatov Institute, Akademika Kurchatova pl. 1, Moscow; 123182, Russia^4;Institute of Cell Biophysics, Institutskaya st. 3, Pushchino, Moscow region; 142290, Russia^5;Bauman Moscow State Technical University, 2-ya Baumanskaya St. 5/1, Moscow; 105005, Russia^6

[关键词] Analytical systems;Biological tissues;Colloidal quantum dots;Fluorescent cores;Fluorescent labels;Functionalizations;Synthesis method;Synthetic materials