A new passive system for contamination-free long-distance cryo-transfer of biological tissues

[摘要] Several new analytical techniques require long-distance cryogenic transfer of samples that need to be kept at stable temperatures for long time periods, but also to be additionally contamination-free. In this study we developed a passive transfer system to fulfil those requirements. With 125mL of liquid nitrogen stored, one cryo-sectioned sample was maintained around 120±1 K and a pressure of about 3x10-7mbar for at least 2 hours. With a total transfer weight of 5 Kg this system can be easily handled and carried by any transportation means so that the same sample can be used for different imaging centres located remotely permitting correlative studies.

[发布机构] Laboratory for Biological Geochemistry, Ecole Polytechnique Fédérale de Lausanne, Lausanne; CH-1015, Switzerland^1;Institute of Earth Sciences, University of Lausanne, Lausanne; CH-1015, Switzerland^2;Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge; CB2 0RE, United Kingdom^3;Paul Scherrer Institute, Villigen; CH-5232, Switzerland^4;Institute of Physics of Biological Systems, Ecole Polytechnique Fédérale de Lausanne, Lausanne; CH-1015, Switzerland^5

[效力级别] 物理学 [学科分类] 材料科学（综合）

[关键词] Biological tissues;Contamination-free;Correlative study;Cryo-transfer;Passive systems;Time-periods;Total transfer;Transfer systems