Rapid detection of gelatin in dental materials using attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR)

[摘要] The presence of gelatin is not limited to food products but has also been found in pharmaceuticals. Most dental materials available in Malaysia are imported from other countries and might contain gelatin which is a protein derived either from porcine, bovine or other animal sources. Authentication of gelatin is crucial due to religious and health concerns. Therefore, this study aimed to detect gelatin in dental materials using ATR-FTIR. Forty two samples of dental material were purchased from dental suppliers and detection was done using ATR-FTIR. The spectrum from each sample was compared against standard bovine and porcine gelatin. Experimental dental paste containing bovine and porcine gelatin at concentrations of 5, 10, 15 and 20% were also prepared for quantification analysis. The results showed that gelatin was present in nine out of forty two samples of dental materials but the species of origin was not confirmed. Meanwhile, in the experimental bovine and porcine dental paste, it was seen that as the concentration increased, the intensity of the absorption of Amide group also increased. Thus, ATR-FTIR can be utilized as a reliable tool to detect gelatin in dental materials and other pharmaceuticals.

[发布机构] International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, Kuala Lumpur, Malaysia^1;Department of Paediatric Dentistry, Kulliyah of Dentistry, International Islamic University Malaysia, Pahang, Kuantan, Malaysia^2;Department of Oral Biology, Kulliyyah of Dentistry, International Islamic University Malaysia, Pahang, Kuantan, Malaysia^3

[效力级别] 医药卫生 [学科分类] 卫生学

[关键词] Amide groups;ATR FTIR;Attenuated total reflection Fourier transform infrared spectroscopy;Dental paste;Health concerns;Malaysia;Quantification analysis;Rapid detection