Scanning electron microscopy and swelling test of shrimp shell chitosan and chitosan_RGD scaffolds

[摘要] Shrimp shell chitosan and chitosan-RGD scaffold membranes are produced to be biocompatible with tissue engineering. Nonetheless, their architectural properties have not yet been studied. Analyze the architectural properties of chitosan and chitosan-RGD scaffolds. Analyze pore count and size, interpore distance, and porosity (using SEM testing and ImageJ analysis) and water absorption (using a swelling test). The properties of the chitosan and chitosan-RGD scaffolds were as follows, respectively. The pore counts were 225 and 153; pore size, 171.4 μam and 180.2 μam; interpore distance, 105.7 μam and 101.4 μam; porosity, 22% and 10.2%; and water absorption, 9.1 mgH2O/mgScaffold and 19.3 mgH2O/mgScaffold. The shrimp shell chitosan-RGD membrane scaffold was found to have architectural properties that make it more conducive to use in tissue engineering.

[发布机构] Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia^1

[效力级别] 医药卫生 [学科分类] 卫生学

[关键词] Architectural properties;Interpore distances;Pore counts;Scaffold membranes;Shrimp shells;Swelling test