Effectiveness of anchovy substrate application on decreasing acid solubility of Sprague Dawley rats' tooth enamel (in vivo)

[摘要] Anchovies (Stolephorus insularis), a natural resource of Indonesia, contain fluoride in the form of CaF2and can function as a fluoridation material to prevent dental caries. The aim of this study is to study the effectiveness of anchovy substrate, through food or topical application, in decreasing the acid solubility of tooth enamel. This research used 14 Sprague Dawley rats as subjects divided into the following 5 groups: baseline, experimental feeding, experimental smearing, and their negative controls. After 15 days of anchovy substrate application, lower incisors were extracted and the acid solubility of enamel was analyzed qualitatively and quantitatively using a stereo microscope and a Micro-Vickers Hardness Tester. Analysis of enamel surface destruction and enamel surface microscopic hardness shifting after a 60 sec application of H2PO4(50% concentration) resulted in a decrease in acid solubility of enamel treated with anchovy substrate. This result can be seen with both the chewing and smearing method. S. insularis can be used as an alternative material for fluoridation.

[发布机构] Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia^1

[效力级别] 医药卫生 [学科分类] 卫生学

[关键词] Acid solubility;Alternative materials;Enamel surface;Micro Vickers hardness;Negative control;Sprague-Dawley rats;Stereo-microscopes;Topical application