Design, synthesis, and functional testing of recombinant cell penetrating peptides

[摘要] Cell penetrating peptides (CPP) are one of the most attractive DNA delivery systems currently in development. In this research, in silico CPP development was performed based on a literature study to look for peptides that induce endosome escape, have the ability to bind DNA, and pass through cell membranes and/or nuclear membranes with a final goal of creating a new CPP to be used as a DNA delivery system. We report herein the successful isolation of three candidate CPP molecules, which have all been successfully expressed and purified by NiNTA. One of the determinants of CPP success as a DNA carrier is the ability of the CPP to bind and protect DNA from the effects of nucleases. The DNA binding test results show that all three CPPs can bind to DNA and protect it from the effects of serum nucleases. These three CPP candidates designed in silico and synthesized in the prokaryote system are eligible candidates for further testing of their ability to deliver DNA in vitro and in vivo.

[发布机构] Research Centre for Virology-Based Health Services and Cancer Pathobiology, Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia^1

[效力级别] 医药卫生 [学科分类] 卫生学

[关键词] Cell-penetrating peptide;DNA carrier;DNA delivery system;Functional testing;Literature studies;Nuclear membranes;Recombinant cells;Successful isolation