Multimodal combinational holographic and fluorescence fluctuation microscopy to obtain spatial super-resolution

[摘要] Ways of combination of holographic and super-resolution fluorescent techniques in the same optical scheme are described. The key parameters influencing achievement of maximum possible resolution are considered. The possibility to choose different fluorescence technic for different types of fluorophores without any optical scheme changes is presented. As a result in case of visualization of the samples, which transparent in optical band, three-dimensional super resolution is received that significantly expands possibilities of the noninvasive analysis of biological samples.

[发布机构] Radiophysical Cdepartment, Lobachevsky State University of Nizhni Novgorod, 23 Gagarin avenue, Nizhny Novgorod, Russia^1;Center for Advanced Biomedical Imaging and Photonics, HMS/BIDMC, Harvard University, 330 Brookline Ave, Boston; MA; 02215, United States^2

[关键词] Biological samples;Fluorescence fluctuation;Fluorescent technique;Multi-modal;Noninvasive analysis;Optical bands;Optical scheme;Super resolution