Combined assessment of cyto-, geno-, and embryotoxicity of silica nanoparticles in experiments in vitro
[摘要] A complex of express methods for cyto-, geno-, and embryotoxicity assessment of nanoparticles (NPs), based on the simultaneous use of three models: the cultures of pre- and post-implantation embryos of mice (egg cleavage, blastocyst formation, 1-5 day of development) and rats (head fold-neurulation stages, 30 somite pairs, 9.5-11.5 day of development), as well as human peripheral blood lymphocytes, is developed. Pathogenic (dysmorphogenic) effects of SiO2 NPs in concentrations of 100 and 200 μg/mL on early embryo development processes were established. The cytogenetic methods used for cyto-, geno-, and mutagenicity assessment (micronuclear test under cytokinesis-block conditions, chromosomal aberrations test, and DNA comet assay) revealed no effects of SiO2 NPs in concentrations 200, 100, and 20 μg/mL.
[发布日期] [发布机构] Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency, G/P Kuzmolovsky, Vsevolozhsky District, Leningrad Region; 199663, Russia^1
[效力级别] 医药卫生 [学科分类]
[关键词] Chromosomal aberration;Embryo development;Embryotoxicity;Human peripheral blood;Mutagenicity;Nanoparticle (NPs);Post-implantation;Simultaneous use [时效性]
