The development of sea sponges (Hymeniacidon perlevis and Amorphinopsis sp) as novel models for genotoxicity assessment and environmental monitoring of pollutants in the aquatic environment

[摘要] The measurement of chemical levels of pollutants and their Biomarkers in resident sessile biota has been suggested for reliable aquatic monitoring and environmental risk assessment. This thesis proposes the use of the comet assay in sponges as a suitable model to complement analytical methods and other established invertebrate aquatic sentinels in aquatic pollution monitoring and risk assessment. A novel ‘in vivo’ exposure sponge cell model was optimised for assessment of DNA strand breaks (DSB) using the alkaline comet assay and Reactive oxygen species (ROS) formation using 2′,7′-Dichlorodihydrofluorescein diacetate (H2DCF-DA) assay. Concentration dependent increase in DSB and ROS formation were observed in sponge cells exposed to aluminium, cadmium, chromium, and nickel in lab based toxicity testing. Correlation between DSB and ROS formation decreased in the order Al< Cd

[发布机构] University:University of Birmingham;Department:School of Biosciences

[关键词] Q Science;QH Natural history;QH426 Genetics