Lipids and metabolites detected by magnetic resonancespectroscopy as biomarkers in nervous system tumour celllines

[摘要] Nuclear magnetic resonance spectroscopy (NMR) resonances from lipids and metabolites in tumours are associated with tumour grade and treatment response.The origin of NMR lipid signal is mainly considered to be cytoplasmic lipid droplets (LDs). The aim of this study is to investigate the lipid species of LDs in nervous system tumour cells and identify potential lipidic or metabolic markers in treatment response. NMR spectroscopic analysis revealed that the LDs contain phosphatidylcholine, cholesterol and cholesterol ester with saturated, mono-unsaturated and polyunsaturated fatty acid species. Both saturated and unsaturated lipids are accumulated into LDs in cancer cell death. It is shown that Uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) and Uridine diphosphate N-acetylglucosamine galactosamine (UDP-GalNAc), the main donors of glycosylation, in parallel with 1H NMR detected lipids, increased in apoptotic cancer cells.LDs in nervous system cancer cell lines contain specific lipid species. To the best of our knowledge, it is the first study mechanistically links UDP-GlcNAc and UDP-GalNAc to cancer cell death.

[发布机构] University:University of Birmingham;Department:Institute of Cancer Studies

[关键词] R Medicine;RC Internal medicine;RC0254 Neoplasms. Tumors. Oncology (including Cancer)