Micro system comprising 96 micro valves on a titer plate

[摘要] A system of 96 micro valves has been developed and mounted on top of a 48-well micro titer plate providing two valves for each well controlling its air inlet and outlet. Testing of the valve system showed that all valves are working and are opened and closed reliably. A pneumatic system is switching inlet and outlet valves independently of each other. The geometry of the feed channels ensures an equal air flow through all wells, when the valves are open. Between the micro valves, one optical fibre was inserted through the lid of each well allowing measuring the oxygen partial pressure in the enclosed air volume by fluorescence sensor spots. Escherichia coli bacteria were grown inside the wells and their metabolism was observed by the oxygen partial pressure change due to respiration. In all 48 wells, the same oxygen transfer rate was observed within an averaged standard deviation of 1 mmol/L/h. The oxygen transfer rate differences compared to a macroscopic standard shake flask system were overall compatible within their uncertainties.

[发布机构] RWTH Aachen University, Aachen; 52074, Germany^1;Konstruktion und Entwicklung von Mikrosystemen, Steinbachstraße 53 B, KEmikro, Germany^2;Aachener Verfahrenstechnik, AVT, Biochemical Engineering, Worringer Weg 1, Germany^3

[效力级别] 力学 [学科分类] 力学，机械学

[关键词] Escherichia coli bacteria;Feed channel;Fluorescence sensors;Oxygen partial pressure;Oxygen transfer rate;Pneumatic system;Standard deviation;Valve systems